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We aim to reduce the number of animals used in experiments as much as possible and use tribromoethanol-based anesthetics, administering 300–500 µl per animal via intraperitoneal injection for anesthesia and their heads were secured in a prone position using a stereotaxic frame

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After coronary artery ligation in mice, intraperitoneal Tβ4 treatment upregulated cardiac ILK and Akt activity, enhanced early myocyte survival, and improved cardiac function — establishing the mechanistic basis for the cardiac and broader tissue-repair claims associated with thymosin beta-4 and its 17–23 fragment TB-500