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These include pre-stored mediators in MC granules, such as histamine and proteases, as well as de novo synthesized mediators such as cytokines and eicosanoids [14]

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Immunohistochemical analysis and section staining Mice in the various treatment groups were anesthetized with pentobarbital sodium (Sigma-Aldrich, USA) and perfused with phosphate-buffered saline (PBS), followed by 4% paraformaldehyde in PBS