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MTT assay Cells were plated in 96-well plates at 2.000 cells per well and 24-well plates at 1 X 10 4 cells per well in complete DMEM (20 mM glucose, 2 mM glutamine, 10% dFBS

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ESR1 locates in epithelial and muscle cells of the uterus and vagina, epithelial and stromal cells of the breast, germinal epithelium of the ovary, and testicular interstitial cells (Pelletier and El-Alfy, 2000)