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Subsequently, the cultures, in a volume of 50 µL, were inoculated using a spot inoculation method onto solid media formulated with the following components: 0.5% tryp-tone, 0.5% yeast extract, 0.5% meat extract, 0.25% NaCl, 0.05% glucose, 0.1% Tween 80, 0.02% MgSO 4 , 0.005% MnSO 4 , 0.004% FeSO 4 , 0.2% ammonium citrate, 0.001% thia-mine, 0.2% K 2 HPO 4 , 0.01% CaCO 3 , 0.005% pyridoxal-5-phosphate, and 1.5% agar