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The plasmid harbors components of a core type IVB secretion/conjugation system complex often used for horizontal propagation, including the gene encoding the conserved central component of the DNA transport activity core complex (Mchl_5595), and traD (Mchl_5572) which lies within a traGDCA gene cluster (Mchl_5572–5575) conserved in other Alphaproteobacteria plasmids including Agrobacterium tumefaciens Ti plasmids (encoded proteins TraG, D, C and A sharing 43, 53, 38 and 44% aa Id, respectively)