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Paramagnetic relaxation enhancement (PRE) experiments were performed using a Bruker 600 MHz NMR spectrometer with a cryogenic probe (GIST, Gwangju)

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How to Recognize Failure: Contamination and Degradation Signs Visible red flags (discard immediately if any appear): cloudiness or turbidity in the solution, visible particles or fibers, a color change from clear (even faint yellow or brown tint), or any precipitate at the bottom of the vial