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The resulting protein pellets were dissolved in 200 µL of 8 M urea in 50 mM Tris/HCl (pH 8.0) and heat-denatured at 95°C for 10 min

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Despite these indications, no studies using in vivo imaging of human brain tissues have examined astrocyte activation, changes in the E/I balance in autism and its relationship with the dopaminergic nervous system